Van den Boom D, Ehrich M Mass spectrometric analysis of cytosine methylation by base-specific cleavage and primer extension methods. We next incorporated the novel homogenization method used for sperm RNA isolation developed by Krawetz and colleagues that utilizes 0. Genomic imprinting in disruptive spermatogenesis. Recent genetic studies have shown a positive association between germline de novo mutations and paternal age 1 — 4. Abnormal methylation of imprinted genes in human sperm is associated with oligozoospermia. Supplementary Material Supplement Click here to view. Taken together, the benefits of our improved method have important implications for settings where sample processing constraints exist. Lastly, our protocol can be extended for isolation of sperm DNA in other mammalian species. Reducing agents TCEP final concentration:
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